Toxic Effect of High Glucose on Cardiomyocytes, H9c2 Cells: Induction of Oxidative Stress and Ameliorative Effect of Trolox

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Ravichandra Shivalingappa Davargaon, . and Asha Devi Sambe, . and Subramanyam Muthangi, V V (2019) Toxic Effect of High Glucose on Cardiomyocytes, H9c2 Cells: Induction of Oxidative Stress and Ameliorative Effect of Trolox. Journal of Biochemical and Molecular Toxicology, 33 (4). ISSN 1099-0461

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Abstract

Oxidative stress (OS) has been implicated in a variety of pathological conditions, including diabetes mellitus, characterized by hyperglycemia. In the present study, OS induced by hyperglycemia and the effect of trolox, a vitamin E analog, were studied in cardiomyocytes and H9c2 cells exposed to 15 to 33 mM glucose (HG) for 24 to 72 hours in Dulbecco modified Eagle medium. Cells treated wirh 24 or 33 mM glucose for 24 hours or above showed decreased viability and adenosine triphosphate (ATP) content with a concomitant increase in radicals of oxygen species, calcium (Ca2+), mitochondrial permeability transition, and oxidative markers, confirming that the cells were under stress. However, upon exposure to 15 mM glucose for 24 hours, H9c2 cells maintained homeostasis and ATP generation. Pretreatment of cells with trolox reduced HG-induced OS to control levels. Here, we report that the toxic effect of HG is highly regulated and that OS induction can be prevented with Trolox, a potential inhibitor of membrane damage.

Item Type: Article
Uncontrolled Keywords: Cardiomyocytes;Cellular Adenosine Triphosphate (ATP);Hyperglycemia;Intracellular Ca2+ (Ca2+)i;Mitochondrial Permeability Transition (MPT)
Subjects: Faculty of Science > Environmental and Biological Sciences > Biological Sciences
Divisions: Jnana Bharathi / Central College Campus > Department of Biological Sciences
Depositing User: Madhu C C
Date Deposited: 13 Oct 2020 10:07
Last Modified: 13 Oct 2020 10:07
URI: http://eprints-bangaloreuniversity.in/id/eprint/9235

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