Trolox Prevents High Glucose-Induced Apoptosis in Rat Myocardial H9c2 Cells by Regulating Glut-4 and Antioxidant Defense Mechanism

Ravichandra Shivalingappa Davargaon, . and Asha Devi Sambe, . and Subramanyam Muthangi, V.V. (2019) Trolox Prevents High Glucose-Induced Apoptosis in Rat Myocardial H9c2 Cells by Regulating Glut-4 and Antioxidant Defense Mechanism. IUBMB Life, 71 (12). p. 1876. ISSN 15216551

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Official URL: https://doi.org/10.1002/iub.2133

Abstract

Redox imbalance due to hyperglycemia is a causative factor for an increased generation of reactive oxygen species (ROS) that leads to mitochondrial dysfunction and the release of cytochrome-c. The aim of the present study is to elucidate the functional role of oxidative stress (OS) in the induction of apoptosis in H9c2 cells in the hyperglycemic state through glucose transporter-4 (GLUT-4) regulation and antioxidant status. H9c2 cells were incubated with 15, 24, and 33 mM glucose for 24, 48, and 72 hr to induce hyperglycemic stress. Hyperglycemic episodes have significantly influenced GLUT-4 mRNA regulation, depleted glutathione (GSH) and its associated enzymes, reduced cellular antioxidant enzymes (AOEs), caused nuclear condensation, and induced apoptosis by activating caspase-9 and 3 and annexin V binding in a concentration and duration-dependent manner. Trolox pretreatment significantly enhanced the GLUT-4 mRNA and antioxidant defense mechanism, suppressed nuclear condensation, and prevented cytochrome-c release, thereby reducing mitochondrial-dependent apoptosis. The present study shows that the toxic effect of high glucose is significantly regulated and that OS induction can be prevented through a water-soluble vitamin E analog “Trolox” treatment.

Item Type: Article
Uncontrolled Keywords: Apoptosis; Hyperglycemia; Oxidative Stress; Rat Myocardial Cells
Subjects: Faculty of Science > Environmental and Biological Sciences > Sericulture
Divisions: Jnana Bharathi / Central College Campus > Department of Sericulture
Depositing User: Madhu C C
Date Deposited: 01 Sep 2020 05:59
Last Modified: 01 Sep 2020 05:59
URI: http://eprints-bangaloreuniversity.in/id/eprint/9072

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