Aldo-keto reductase-1 (AKR1) protect cellular enzymes from salt stress by detoxifying reactive cytotoxic compounds

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Vemanna, R.S. and Babitha, K.C. and Solanki, J.K. and Amarnatha Reddy, V. and Sarangi, S.K. and Udayakumar, M. (2017) Aldo-keto reductase-1 (AKR1) protect cellular enzymes from salt stress by detoxifying reactive cytotoxic compounds. Plant Physiology and Biochemistry, 113. pp. 177-186. ISSN 09819428

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Official URL: http://doi.org/10.1016/j.plaphy.2017.02.012

Abstract

Cytotoxic compounds like reactive carbonyl compounds such as methylglyoxal (MG), melandialdehyde (MDA), besides the ROS accumulate significantly at higher levels under salinity stress conditions and affect lipids and proteins that inhibit plant growth and productivity. The detoxification of these cytotoxic compounds by overexpression of NADPH-dependent Aldo-ketoreductase (AKR1) enzyme enhances the salinity stress tolerance in tobacco. The PsAKR1 overexpression plants showed higher survival and chlorophyll content and reduced MDA, H2O2, and MG levels under NaCl stress. The transgenic plants showed reduced levels of Na+ levels in both root and shoot due to reduced reactive carbonyl compounds (RCCs) and showed enhanced membrane stability resulted in higher root growth and biomass. The increased levels of antioxidant glutathione and enhanced activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) suggest AKR1 could protect these enzymes from the RCC induced protein carbonylation by detoxification process. The transgenics also showed higher activity of delta 1-pyrroline-5- carboxylate synthase (P5CS) enzyme resulted in increasedproline levels to maintain osmotic homeostasis. The results demonstrates that the AKR1 protects proteins or enzymes that are involved in scavenging of cytotoxic compounds by detoxifying RCCs generated under salinity stress. © 2017 Elsevier Masson SAS

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: aldehyde dehydrogenase; aldehyde reductase; antioxidant; ascorbate peroxidase; carbonyl reductase; chlorophyll; glutathione reductase; hydrogen peroxide; methylglyoxal; ornithine oxoacid aminotransferase; oxidoreductase; proline; superoxide dismutase, biomass; biosynthesis; enzymology; genetics; halophyte; metabolism; osmotic pressure; photosynthesis; physiological stress; physiology; salinity; tobacco; transgenic plant, Aldehyde Dehydrogenase; Aldehyde Reductase; Antioxidants; Ascorbate Peroxidases; Biomass; Chlorophyll; Glutathione Reductase; Hydrogen Peroxide; Ornithine-Oxo-Acid Transaminase; Osmotic Pressure; Oxidoreductases; Photosynthesis; Plants, Genetically Modified; Proline; Pyruvaldehyde; Salinity; Salt-Tolerant Plants; Stress, Physiological; Superoxide Dismutase; Tobacco
Subjects: Faculty of Science > Environmental and Biological Sciences > Microbiology & Biotechnology
Divisions: Jnana Bharathi / Central College Campus > Department of Microbiology and Biotechnology
Depositing User: Dr. M Anjanappa
Date Deposited: 13 Jun 2018 06:28
Last Modified: 13 Jun 2018 06:28
URI: http://eprints-bangaloreuniversity.in/id/eprint/8191

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