Somatic embryogenesis in flax


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Tejavathi, D.H. and Lakshmi Sita, G. and Sunita, A.T. (2000) Somatic embryogenesis in flax. Plant Cell, Tissue and Organ Culture, 63 (2). pp. 155-159. ISSN 1573-5044

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Callus was initiated from hypocotyl explants of three elite varieties of Linum usitatissimum L. on Murashige and Skoog (MS) medium supplemented with various growth regulators. Somatic embryos differentiated from callus grown on MS + α-naphthaleneacetic acid. The origin from a single cell and subsequent developmental stages was traced. Embryos developed into normal plants upon transfer to MS + 2.69 μM α-naphthaleneacetic acid + 2.22 μM 6-benzyladenine in two varieties. The medium was supplemented with several additives (abscisic acid, glycine, lysine, glutamine and casein hydrolysate) to increase the percentage of normal plant development from embryos; none of the additives could promote the growth of the shoot apex.

Item Type: Article
Additional Information: cited By 17
Uncontrolled Keywords: Linum usitatissimum
Subjects: Faculty of Science > Environmental and Biological Sciences > Botany
Divisions: Jnana Bharathi / Central College Campus > Department of Botany
Depositing User: Mr. Kirana Kumar D
Date Deposited: 08 Feb 2016 07:18
Last Modified: 08 Feb 2016 07:18

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