Co-optimization of xyloglucanase and beta-glucosidase using response surface methodology

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Rashmi, R. and Siddalingamurthy, K.R. (2015) Co-optimization of xyloglucanase and beta-glucosidase using response surface methodology. JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH, 74 (8). pp. 450-456. ISSN 0022-4456

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Official URL: http://nopr.niscair.res.in/handle/123456789/31934

Abstract

Xyloglucanase and beta-glucosidase are important accessory enzymes required for hydrolysis of lignocellulosic biomass. In an effort to reduce the cost of production, tamarind kernel polysaccharide was used as the substrate for production of both enzymes. Further, enhancement of enzymes' production was carried out using Plackett-Burman design and a relatively new methodology, viz, ridge analysis. The results indicate that the tamarind kernel polysaccharide supported production of both enzymes significantly. By statistical analysis, significant media components were identified and optimized. As a result of this study, there was 2.1 and 9.6 fold increase in production of xyloglucanase and beta-glucosidase respectively. This study is the first report on the optimization of xyloglucanase production

Item Type: Article
Subjects: Faculty of Science > Environmental and Biological Sciences > Biochemistry
Divisions: Jnana Bharathi / Central College Campus > Department of Biochemistry
Depositing User: Ms. Deepa M Kolli
Date Deposited: 24 Oct 2016 09:14
Last Modified: 24 Oct 2016 09:14
URI: http://eprints-bangaloreuniversity.in/id/eprint/6908

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