Diversity of Drimia indica (Roxb.) Jessop and its relationship to Drimia nagarjunae using phenotypic traits and molecular markers.

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Narendranath Alluri, . and Shivakameshwari, M.N. and Manohar, S.H. and Mala Majumdar, . (2015) Diversity of Drimia indica (Roxb.) Jessop and its relationship to Drimia nagarjunae using phenotypic traits and molecular markers. Indian Journal of Experimental Biology, 53. pp. 412-416. ISSN 0975-1009

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Official URL: http://imsear.hellis.org/handle/123456789/158525

Abstract

In the present study, we explored the diversity of different accessions of Drimia indica and its relation to D. nagarjunae using phenotypic traits and molecular markers. Twenty populations of D. indica, from different parts of India, were compared with D. nagarjunae, an endangered medicinal plant collected from Andhra Pradesh, India. Two species showed appreciable phenotypic diversity in number of leaves, leaf indices, bulb circumference, bulb length and length of roots. The principal component analysis (PCA) performed on above 5 quantitative characters to determine relationship among populations, has distinguished D. nagarjunae from D. indica phenotypically. Genetic diversity was analysed using RAPD and ISSR primers which produced reproducible bands in 8 RAPD and 3 ISSR primers. A total of 89 amplicons were observed, of which 69 (77.53 %) were polymorphic. Cluster diagram and phylogenetic linkage showed that D. nagarjunae formed a separate cluster, showing no similarity with any of the populations of D. indica. The molecular marker data correlated with PCA of phenotypic traits. Current investigations have demonstrated that the statistical approach for phenotypic characters and molecular markers analysis can be applied to study diversity in Drimia species.

Item Type: Article
Uncontrolled Keywords: Indian squill Population relationship Species diversity Squill White squill
Subjects: Faculty of Science > Environmental and Biological Sciences > Botany
Divisions: Jnana Bharathi / Central College Campus > Department of Botany
Depositing User: Ms. Sathyavathi N
Date Deposited: 22 Oct 2016 08:38
Last Modified: 22 Oct 2016 08:38
URI: http://eprints-bangaloreuniversity.in/id/eprint/6134

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