Rapid callus induction and regenerative studies in shoot tip explants of Withania somnifera (L.) dunal-​indian ginseng

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Raveesha, H.R. and Manjunathareddy, G.R. (2014) Rapid callus induction and regenerative studies in shoot tip explants of Withania somnifera (L.) dunal-​indian ginseng. International Journal of Biology, Pharmacy and Allied Sciences, 3 (11). pp. 2561-2568. ISSN 2277-4998

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Abstract

The efficient large scale in vitro regeneration protocol was developed by indirect organogenesis of Withania somnifera (L.) Dunal. In vivo Shoot tip explants of 2-​3 mo old seedlings were grown on Murashige and Skoogs (MS) medium supplemented with various concns. of different auxins (1-​4 mgl-​1) and cytokinins (0.5-​1 mgl-​1)​. The shoot initiation was obsd. when the callus were sub cultured on MS medium contg. different concn. of cytokinins (1-​4 mgl-​1) and low concn. of Auxins (0.5-​1 mgl-​1)​. Shoots derived from callus in primary cultures were transferred to proliferation medium (multiple shoot regeneration) of different cytokinins. Rapid shoot multiplication was obsd. on MS medium supplemented with KN (2 mgl-​1) and BA (0.5 mgl-​1)​. Shoots were rooted on MS medium supplemented with 1 mgl-​1 of IBA. Plantlets with well developed roots were transferred to sterile soil mixt. The pots were maintained in green house condition and transferred to field.

Item Type: Article
Uncontrolled Keywords: Shoot tip, Withania somnifera, Auxins, Cytokinins, Proliferation medium.
Subjects: Faculty of Science > Environmental and Biological Sciences > Botany
Divisions: Jnana Bharathi / Central College Campus > Department of Botany
Depositing User: Ms. Sathyavathi N
Date Deposited: 29 Aug 2016 09:36
Last Modified: 29 Aug 2016 09:36
URI: http://eprints-bangaloreuniversity.in/id/eprint/5026

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