Evaluation of appropriate reference gene for normalization of microRNA expression by real-​time PCR in Lablab purpureus under abiotic stress conditions

Thilagavath, . and Adikeshavan, . and Devara, . and Varadahally, R. (2016) Evaluation of appropriate reference gene for normalization of microRNA expression by real-​time PCR in Lablab purpureus under abiotic stress conditions. Biologia (Berlin, Germany), 71 (6). pp. 660-668. ISSN 0006-3088

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Official URL: https://doi.org/10.1515/biolog-2016-0091

Abstract

MicroRNAs (miRNAs) play key roles in plant responses to biotic and abiotic stresses by modulating their own expression and a wide array of target mRNAs. Reverse transcription quant. real-​time PCR is a sensitive and widely used method to study miRNA expression profile. Accurate anal. and interpretation of results require the selection of an appropriate ref. gene. Ref. genes selected should have a const. expression level under different stress conditions. Six ref. candidates, including two miRNAs (miRNA 156 and miRNA 172)​, an rRNA (5S rRNA)​, a snRNA (U6) and two protein coding genes (actin and protein phosphatase 2A)​, were selected for normalization of miRNA expression in Lablab purpureus. The expression stability of candidate ref. genes was investigated in ten samples and analyzed using NormFinder, BestKeeper and hkgFinder softwares. The analyses suggested that miRNA 156 is the appropriate ref. gene, as it had better expression stability than protein-​coding genes, and other non-​coding RNAs.

Item Type: Article
Uncontrolled Keywords: Lablab purpureus; microRNA; RT-qPCR; reference genes; internal control for qPCR; abiotic stress
Subjects: Faculty of Science > Environmental and Biological Sciences > Biochemistry
Divisions: Jnana Bharathi / Central College Campus > Department of Biochemistry
Depositing User: Mr . Chandrashekar
Date Deposited: 18 Aug 2016 10:16
Last Modified: 18 Aug 2016 10:16
URI: http://eprints-bangaloreuniversity.in/id/eprint/4718

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