High Frequency Shoot Multiplication and Callus Regeneration of Turmeric

Roopadarshini, V. (2010) High Frequency Shoot Multiplication and Callus Regeneration of Turmeric. International Journal of Biotechnology & Biochemistry, 6 (5). pp. 723-733. ISSN 0974-4762

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Official URL: http://www.ripublication.com/Volume/ijbbv6n5.htm

Abstract

An efficient protocol for shoot multiplication and callus regeneration has been standardized in an important spice plant, Curcuma longa L. (turmeric) variety Suguna. LSBM fortified with BAP (3.0 mgl-1) was found to be the suitable medium for initiation and multiplication of shoots (1:36) from the in vivo vegetative bud explant. Profuse, compact creamish white callus was induced and proliferated from in vivo vegetative bud explant on LSBM fortified with 2,4-D (3.0 mgl-1). Further, LSBM fortified with BAP (3.5 mgl-1) was found to be the best medium for initiation, multiplication and retrieval of complete plantlets from vegetative bud callus when compared to other concentrations of BAP tried. The shoots obtained via multiple shoot induction and callus regeneration were rooted on the same medium thereby eliminating an additional step for rooting. The complete plantlets were transferred to the field with 95% survival frequency.a

Item Type: Article
Subjects: Faculty of Science > Environmental and Biological Sciences > Botany
Divisions: Jnana Bharathi / Central College Campus > Department of Botany
Depositing User: Mrs Rajani S
Date Deposited: 14 Jul 2016 07:12
Last Modified: 14 Jul 2016 07:12
URI: http://eprints-bangaloreuniversity.in/id/eprint/3934

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