Expression of miRNAs Confers Enhanced Tolerance to Drought and Salt Stress in Finger Millet (Eleusine coracona)

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Nageshbabu, R. and Usha, . and Jyothi, M.N. and Sharadamma, N. (2013) Expression of miRNAs Confers Enhanced Tolerance to Drought and Salt Stress in Finger Millet (Eleusine coracona). Journal of Stress Physiology and Biochemistry, 9 (3). pp. 220-231. ISSN 1997-0838

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Abstract

Plants respond to the environmental cues in various ways, recent knowledge of RNA interference in conferring stress tolerance had become a new hope of developing tolerant varieties. Here we attempt to unfold the molecular mechanism of stress tolerance through miRNA profiling and expression analysis in Finger millet (Eleusine coracona) under salt and drought stress conditions. The expression analysis of 12 stress specific conserved miRNAs was studied using semi-quantitative real time PCR and Northern blot assay. Our studies revealed that, although most of the miRNAs responded to the stresses, the expression of particular miRNA differed with the nature of stress and the tissue. The expression analysis was correlated with the existing data of their target genes. Abiotic stress up-regulated miRNAs are expected to target negative regulators of stress responses or positive regulators of processes that are inhibited by stresses. On the other hand, stress down-regulated miRNAs may repress the expression of positive regulators and/or stress up-regulated genes. Thus the current study of miRNAs and their targets under abiotic stress conditions displays miRNAs may be good candidates to attribute the stress tolerance in plants by transgenic technology.

Item Type: Article
Uncontrolled Keywords: Drought; Finger Millet; Mirna; Salt Stress
Subjects: Faculty of Science > Environmental and Biological Sciences > Biochemistry
Divisions: Jnana Bharathi / Central College Campus > Department of Biochemistry
Depositing User: Mr. Narayanaswamy B V
Date Deposited: 01 Jul 2016 07:33
Last Modified: 01 Jul 2016 07:33
URI: http://eprints-bangaloreuniversity.in/id/eprint/3739

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