Esterases as Genetic Markers in Finger Millet


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Aravinda Upadhya, . and Lakshmi, K.G. and Veerabhadrappa, P.S. (1985) Esterases as Genetic Markers in Finger Millet. Journal of the Science of Food and Agriculture, 36 (5). pp. 319-325. ISSN 1097-0010

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Different varieties of finger millet (Eleusine coracana Gaertn.) were screened for esterase activity colorimetrically and electrophoretically using 1-naphthyl acetate and acetylthiocholine chloride as substrates. The Indian brown seed coat variety (Purna), the Indian white seed coat variety (Hamsa), hybrids of these designated as HPB (brown) and HPW (white), African varieties (brown) and Indian-African hybrid varieties (brown) all exhibited 1-naphthyl acetate hydrolysing activity and showed 6,5,6,5,8 and 8 esterolytic bands respectively on gel electrophoresis. The white seed coat varieties, both parental (Hamsa) and hybrid (HPW), did not possess any acetylthiocholine chloride hydrolysing activity while all the brown seed coat varieties did, the African varieties having greater activities than Indian brown seed coat varieties. Thus, the demonstrable variation in esterase isozymic pattern and cholinester hydrolysing activity with the varieties tested provides a useful genetic marker for identifying different varieties of finger millet.

Item Type: Article
Uncontrolled Keywords: Esterases;Eleusine coracana Gaertn;genetic markers
Subjects: Faculty of Science > Pure Sciences > Chemistry
Divisions: Jnana Bharathi / Central College Campus > Department of Chemistry
Depositing User: Mrs Sushma H R
Date Deposited: 26 May 2016 07:51
Last Modified: 26 May 2016 07:51

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