Novel ligand 1-Benzyl-3-(4-ethyl-pyridin-2-yl)-thiourea and Cu (I) complexes: DNA interaction, antibacterial and thermal studies


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Chetana, P.R. and Srinatha, B.S. and Somashekar, M.N. and Policegoudra, R.S. and Rao, R. and Maity, B. and Aradhya, S.M. (2013) Novel ligand 1-Benzyl-3-(4-ethyl-pyridin-2-yl)-thiourea and Cu (I) complexes: DNA interaction, antibacterial and thermal studies. International Journal of Pharmaceutical Sciences Review and Research, 21 (1). pp. 355-363. ISSN 0976044X

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Novel Ligand 1-Benzyl-3-(4-Ethyl-Pyridin-2-Yl)-Thiourea and Cu (I) Complexes DNA Interaction,.pdf
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The new ligand 1-Benzyl-3-(4-ethyl-pyridin-2-yl)-thiourea (1), and two copper(I) complexes Cu(4ETU)2Cl (2), Cu(4ETU)(B)Cl (3) where 4ETU=1-Benzyl-3-(4-ethyl-pyridin-2-yl)-thiourea, B is a N,N-donor heterocyclic base, viz. 1,10-phenanthroline (phen, 3), were synthesized, characterized by various physico-chemical and spectroscopic techniques. The elemental analysis suggests that the stoichiometry to be 1:2 (metal:ligand) for 2 and 1:1:1 (metal:ligand:B) for 3. PXRD analysis provides the crystalline nature of the metal complexes. IR data coupled with electronic spectra and molar conductance values suggest that the complex 2 shows the presence of a trigonal planar geometry and the complex 3 shows the presence of a tetragonal geometry about the Cu(I) centre. Binding interactions of the complexes with calf thymus (CT) DNA have been investigated by absorption, emission, DNA thermal denaturation and viscosity studies. The phen complex displays significant binding propensity to the CT DNA giving an order: 3 (phen) > 2. Complex 3 shows efficient oxidative cleavage of SC-DNA in the presence of H2O2 involving hydroxyl radical species as evidenced from the control data showing inhibition of DNA cleavage in the presence of DMSO and KI. The compounds were screened for their in vitro anti-bacterial activity using Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus) and Gramnegative bacteria (Esherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia). Complexes 2 & 3 exhibits higher antibacterial activity against Gram-positive bacteria than the Gram-negative bacteria. Antibacterial activity is higher when thiourea coordinates to metal ion than the thiourea alone. The thermogram of complex 2 exhibits single step and complex 3 exhibits two step decomposition behavior.

Item Type: Article
Additional Information: cited By 3
Uncontrolled Keywords: 1 benzyl 3 (4 ethyl pyridin 2 yl) thiourea; copper 1 benzyl 3 (4 ethyl pyridin 2 yl) thiourea (1,10 phenanthroline) chloride; copper 1 benzyl 3 (4 ethyl pyridin 2 yl) thiourea chloride; copper complex; dimethyl sulfoxide; DNA; heterocyclic compound; hydrogen peroxide; hydroxyl radical; ligand; metal; metal complex; metal ion; thiourea derivative; unclassified drug, absorption spectroscopy; antibacterial activity; article; Bacillus subtilis; complex formation; DNA cleavage; DNA denaturation; drug decomposition; drug DNA interaction; drug screening; drug structure; drug synthesis; electric conductivity; Escherichia coli; flame photometry; geometry; in vitro study; infrared spectroscopy; Klebsiella pneumoniae; Micrococcus luteus; nonhuman; oxidative stress; physical chemistry; proton nuclear magnetic resonance; Pseudomonas aeruginosa; Staphylococcus aureus; stoichiometry; structure analysis; thermal analysis; thermography; thymus; viscosity; X ray diffraction
Subjects: Faculty of Science > Pure Sciences > Chemistry
Divisions: Jnana Bharathi / Central College Campus > Department of Chemistry
Depositing User: Mr. Kirana Kumar D
Date Deposited: 11 Apr 2016 07:39
Last Modified: 11 Apr 2016 07:39

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