Expression of a synthetic cry1AcF gene in transgenic Pigeon pea confers resistance to Helicoverpa armigera


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Ramu, S.V. and Rohini, S. and Keshavareddy, G. and Gowri Neelima, M. and Shanmugam, N.B. and Kumar, A.R.V. and Sarangi, S.K. and Ananda Kumar, P. and Udayakumar, M. (2012) Expression of a synthetic cry1AcF gene in transgenic Pigeon pea confers resistance to Helicoverpa armigera. Journal of Applied Entomology, 136 (9). pp. 675-687. ISSN 09312048

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Pigeon pea is an important legume. Yield losses due to insect pests are enormous in the cultivation of this crop. Expression of cry proteins has led to increased resistance to pests in several crops. We report in this paper, expression of a chimeric cry1AcF (encoding cry1Ac and cry1F domains) gene in transgenic pigeon pea and its resistance towards Helicoverpa armigera. PCR, Southern hybridization, RT-PCR and Western analysis confirmed stable integration and expression of the cry1AcF gene in pigeon pea transgenics. When screened for efficacy of the transformants for resistance against H. armigera, the transgenics showed not only high mortality of the larva but could also resist the damage caused by the larvae. Analysis for the stable integration, expression and efficacy of the transgenics resulted in the identification of four T3 plants arising from two T1 backgrounds as highly promising. The results demonstrate potentiality of the chimeric cry1AcF gene in developing H. armigera-resistant pigeon pea. © 2011 Blackwell Verlag, GmbH.

Item Type: Article
Additional Information: cited By 4
Uncontrolled Keywords: bacterium; gene expression; genetic analysis; legume; mortality; moth; pest species; polymerase chain reaction; protein; transformation; transgenic plant; yield response, Agrobacterium; Cajanus cajan; Helicoverpa armigera; Hexapoda; Rhizobium
Subjects: Faculty of Science > Environmental and Biological Sciences > Microbiology & Biotechnology
Divisions: Jnana Bharathi / Central College Campus > Department of Microbiology and Biotechnology
Depositing User: Mr. Kirana Kumar D
Date Deposited: 02 Apr 2016 07:14
Last Modified: 02 Apr 2016 07:14

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