Molecular strain typing of Wolbachia infection from Indian mosquitoes using wsp gene

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Ravikumar, H. and Ramachandraswamy, N. and Puttaraju, H.P. (2011) Molecular strain typing of Wolbachia infection from Indian mosquitoes using wsp gene. Asian Pacific Journal of Tropical Disease, 1 (2). pp. 106-109. ISSN 22221808

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Official URL: https://doi.org/10.1016/S2222-1808(11)60046-7

Abstract

Objective: To determine the status of Wolbachia subgroup and phylogenetic relationships in Indian mosquitoes. Methods: Recently we reported Wolbachia infection in eight out of twenty field-caught mosquito species of India, using wsp specific primers. DNA extracted from these mosquito species were used for PCR amplification and sequencing. Results: Wolbachia A harboured in Aedes albopictus and Culex gelidus belongs to the subgroup AlbA whereas Wolbachia B harboured in Aedes albopictus and Culex quinquefasciatus belongs to the subgroup Pip and of Culex vishnui belongs to subgroup Con. However, Wolbachia harboured in Armigeres subalbatus, Armigeres kesseli, Culex sitiens and Toxorhynchites splendens could not be placed into any known subgroup and may represent other unknown strains of Wolbachia. Our phylogenetic analysis revealed eight novel Wolbachia strains, four in the A group and four in the B group. Most of the Wolbachia strains present in Indian mosquitoes belong to the Albo, Pip and Con groups. Conclusions: The similarities and differences between Wolbachia strains infecting different mosquito species are fundamental for estimating how easily mosquitoes acquire new infections. © 2011 Asian Pacific Tropical Medicine Press.

Item Type: Article
Additional Information: cited By 15
Uncontrolled Keywords: DNA, Aedes albopictus; animal experiment; Armigeres kesseli; armigeres subalbatus; article; controlled study; Culex; culex gelidus; Culex quinquefasciatus; culex sitiens; Culex vishnui; disease carrier; DNA extraction; gene; gene amplification; gene sequence; India; molecular typing; mosquito; nonhuman; nucleotide sequence; phylogenetic tree; phylogeny; polymerase chain reaction; priority journal; strain difference; Toxorhynchites splendes; Wolbachia; wsp gene
Subjects: Faculty of Science > Environmental and Biological Sciences > Biochemistry
Divisions: Jnana Bharathi / Central College Campus > Department of Biochemistry
Depositing User: Mr. Kirana Kumar D
Date Deposited: 01 Apr 2016 10:12
Last Modified: 01 Apr 2016 10:12
URI: http://eprints-bangaloreuniversity.in/id/eprint/2484

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