Regeneration of multiple shoots from the callus cultures of Macrotyloma uniflorum (Lam.) Verdc

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Tejavathi, D.H. and Devaraj, V.R. and Murthy, S.M. and Anitha, P. and Nijagunaiah, R. (2010) Regeneration of multiple shoots from the callus cultures of Macrotyloma uniflorum (Lam.) Verdc. Indian Journal of Biotechnology, 9 (1). pp. 101-105. ISSN 09725849

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Abstract

An effective protocol for the regeneration of multiple shoots from the callus, derived from shoot tip and cotyledonary node, was developed for the first time for Macrotyloma uniflorum (Lam.) Verdc. Shoot apices and cotyledonary nodes from 9-d-old aseptically grown seedlings were inoculated onto MS, L 2 and MMS media supplemented with various growth regulators. Proliferation of about 17 shoots was obtained on AS supplemented MS medium after 16 d of culture, while about 14 and 20 shoots regenerated from the callus on MMS1+NAA (1.86 μM)+BAP(0.45 μM) and L2+IBA (0.45 μM)+BAP (0.11 μM), respectively after 30 d of culture. Regenerated shoots were rooted on MS+IAA (0.11 μM)+Kn (0.21 μ) with upto 6 roots per shoot. Rooted shoots were sequentially acclimatized by transferring them to sterile distilled water and tap water before planting in various pot mixtures. A maximum of 60 survival rate was noticed on a mixture of soil:sand:manure (1:1:1).

Item Type: Article
Additional Information: cited By 2
Uncontrolled Keywords: auxin; cytokinin; sodium chloride; tap water; vitamin; water, acclimatization; article; callus culture; controlled study; cotyledon; culture medium; growth regulation; legume; Macrotyloma uniflorum; nonhuman; plant growth; plant regeneration; rooting; seedling; shoot; shoot tip culture; survival rate; tissue growth, Dolichos biflorus; Macrotyloma uniflorum
Subjects: Faculty of Science > Environmental and Biological Sciences > Biochemistry
Faculty of Science > Environmental and Biological Sciences > Botany
Divisions: Jnana Bharathi / Central College Campus > Department of Botany
Jnana Bharathi / Central College Campus > Department of Biochemistry
Depositing User: Mr. Kirana Kumar D
Date Deposited: 28 Mar 2016 07:19
Last Modified: 28 Mar 2016 07:19
URI: http://eprints-bangaloreuniversity.in/id/eprint/2283

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