Assessment of Water quality in Madiwala lake, Bangalore in relation to faecal contamination

Anupama, B. and Jumbe, A.S. and Nandini, N. (2009) Assessment of Water quality in Madiwala lake, Bangalore in relation to faecal contamination. Nature Environment and Pollution Technology, 8 (3). pp. 497-501. ISSN 09726268

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Tanks in and around urban areas of Bangalore receive considerable amount of sewage, which has caused a severe and persistent microbial pollution. The main objective of this study was to measure level of faecal contamination in Madiwala lake using different techniques of E. coli estimation. Results show that the golden green colonies of conforms with metallic sheen showed a luxuriant growth at 35°C with M-Endo Agar (M1106) at incubation time of 24-48 hrs. The growth of both E. coli and Enterobacter aerogens using M-Endo Agar plate was also luxuriant at 35°C temperature. But E. aerogens colonies were pink and mucoid while that of E. coli varied from pink to rose-red with metallic sheen. The confirmatory test using Agar M-392 confirmed the presence of Enterococcus fecalis in yellow colour colonies at luxuriant growth of 35°C temperature. The presumptive test results of different volumes of the lake samples after 24 and 48 hours using lauryl tryptose broth indicated significant colour change, gas production, and turbidity. Confirmed results showed gas production in brilliant green using lactose bile broth at 37°C in 48 hrs. The completed result with EMB agar inoculated with positively confirmed test tube incubated at for 24 hrs at 35°C showed greenish metallic sheen colonies while the coliform colonies inoculated on Nutrient Agar slant and a broth tube incubated for 48 hrs at 35°C showed growth as observed on slant with gas production in the broth tube. Moreover, Gram staining results showed Gram negative with sporing rods in the tested samples. The MPN Index of the lake sample was found to be 38/100mL. Water is generally considered safe for drinking if it contains fewer than 4 coliforms/100 mL. It is also considered safe if it contains less than 2 Enterococcus bacteria colonies/100mL of a sample. These observations indicate that environmental status of Madiwala lake with respect to microbial pollution is continuing to deteriorate.

Item Type: Article
Additional Information: cited By 0
Uncontrolled Keywords: Agar plates; Agar slants; Brilliant green; Colour change; Confirmatory test; E. coli; Enterobacter aerogens; Enterococcus bacteria; Faecal contamination; Gas productions; Gram-staining; Incubation time; Lake water quality; Lauryl tryptose broth; Microbial pollution; Presumptive test; Test tube; Urban areas, Algae; Contamination; Escherichia coli; Gas pipelines; Gas producers; Lakes; Polysaccharides; Potable water; Sewage; Sugars; Turbidity; Water quality, Lake pollution, fecal coliform; lake water; microbial community; sewage; urban area; water pollution; water quality, Bangalore; India; Karnataka; Madiwala Lake, Bacteria (microorganisms); Enterobacter; Enterococcus; Enterococcus faecalis; Escherichia coli; Negibacteria
Subjects: Faculty of Science > Environmental and Biological Sciences > Environmental Science
Divisions: Jnana Bharathi / Central College Campus > Department of Environmental Science
Depositing User: Mr. Kirana Kumar D
Date Deposited: 11 Mar 2016 11:15
Last Modified: 11 Mar 2016 11:15

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