Oxidative stress and intracellular pH in the young and old erythrocytes of rat

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Asha Devi, S. and Shiva Shankar Reddy, C.S. and Subramanyam, M.V.V. (2009) Oxidative stress and intracellular pH in the young and old erythrocytes of rat. Biogerontology, 10 (6). pp. 659-669. ISSN 1573-6768

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Official URL: https://doi.org/10.1007/s10522-009-9212-8

Abstract

The effects of oxidative stress (OS) on the rat erythrocytes (RBCs) that were fractionated on the percoll/BSA gradient into young and old cells were studied to find out if the altered Na +/H + and Cl -/HCO 3 - antiporters and in turn the intracellular pH (pH i) could act as one of the promoters of cell death. Old cells were more spherical with lesser surface area, more fragile osmotically and had lesser protein sulphydryl content than the young cells. OS was induced in RBCs by 2,2â²-azobis (2-amidinopropane) dihydrochloride (AAPH). AAPH increased the superoxide dismutase (SOD) activity and MDA level and, the changes between the young and old. Interestingly, vitamin C was effective in reducing MDA in the old. Further, in the old a rapid Na +-dependent acidification in the presence of AAPH and a marginal acidosis in the presence of vitamin C were evident. Old RBCs exhibited higher acidosis and vitamin C was less effective in lowering the stress-induced acidosis compared to the young. Our studies suggest that increased acidosis followed by low intracellular pH could be one of the determinant factors for the disappearance of old RBCs from circulation, and perhaps of the young too under OS. © 2009 Springer Science+Business Media B.V.

Item Type: Article
Additional Information: cited By 6
Uncontrolled Keywords: 2,2' azobis(2 amidinopropane); ascorbic acid; bicarbonate; malonaldehyde; sodium chloride; sodium hydroxide; sodium ion; superoxide dismutase, acidification; age; animal cell; article; cell death; cell fractionation; cell pH; controlled study; enzyme activity; erythrocyte; erythrocyte fragility; erythrocyte structure; lipid peroxidation; nonhuman; oxidative stress; priority journal; protein content; rat, Amidines; Analysis of Variance; Animals; Antioxidants; Ascorbic Acid; Cell Aging; Chloride-Bicarbonate Antiporters; Erythrocytes; Hydrogen-Ion Concentration; Lipid Peroxidation; Male; Malondialdehyde; Osmotic Fragility; Oxidants; Oxidative Stress; Rats; Sodium-Hydrogen Antiporter; Superoxide Dismutase
Subjects: Faculty of Science > Environmental and Biological Sciences > Sericulture
Faculty of Science > Environmental and Biological Sciences > Zoology
Divisions: Jnana Bharathi / Central College Campus > Department of Sericulture
Jnana Bharathi / Central College Campus > Department of Zoology
Depositing User: Mr. Kirana Kumar D
Date Deposited: 05 Mar 2016 10:55
Last Modified: 05 Mar 2016 10:55
URI: http://eprints-bangaloreuniversity.in/id/eprint/1816

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