Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica

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Deepak, A.V. and Thippeswamy, G. and Shivakameshwari, M.N. and Salimath, B.P. (2003) Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica. Biochemical and Biophysical Research Communications, 311 (3). pp. 735-742. ISSN 0006291X

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Isolation and characterization of a 29-kDa glycoprotein with antifungal activity from bulbs of Urginea indica.pdf

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Official URL: https://doi.org/10.1016/j.bbrc.2003.10.056

Abstract

In this study an antifungal protein from Urginea indica bulbs was purified to homogeneity by acid precipitation, Diol 300 Gel-filtration, and C 18 reverse phase HPLC. Its molecular mass was estimated to be 29kDa and periodic acid-Schiff (PAS) staining showed that identified antifungal molecule is a glycoprotein. The neutralization of antifungal activity after periodate oxidation of 29kDa glycoprotein suggests that the glycan part of the molecule appears to be involved in antifungal activity. N-terminal amino acid sequence of the purified protein was determined as SQLKAXIXDF. This sequence had no sequence similarity with any antifungal proteins. A polyclonal antiserum was raised against purified protein and used in immunolocalization analysis. Results suggest that it is localized to the cell wall of the bulb. Antifungal tests have demonstrated that U. indica protein exerts a fungistatic effect. It completely inhibits the germination of spores and hyphal growth of Fusarium oxysporum. © 2003 Elsevier Inc. All rights reserved.

Item Type: Article
Additional Information: cited By 18
Uncontrolled Keywords: antifungal agent; glycan derivative; glycoprotein; periodate, amino terminal sequence; antifungal activity; article; cell wall; controlled study; drug effect; drug purification; fungus growth; fungus hyphae; Fusarium oxysporum; immunolocalization; molecular weight; nonhuman; periodic acid Schiff stain; plant bulb; priority journal; protein purification; spore germination; urginea indica, Amino Acid Sequence; Animals; Antibodies; Antifungal Agents; Blotting, Western; Carbohydrates; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Fusarium; Glycoproteins; Liliaceae; Molecular Sequence Data; Plant Components; Plant Extracts; Plant Proteins; Protein Structure, Tertiary; Rabbits; Time Factors, Fusarium; Fusarium oxysporum; Urginea
Subjects: Faculty of Science > Environmental and Biological Sciences > Botany
Divisions: Jnana Bharathi / Central College Campus > Department of Botany
Depositing User: Mr. Kirana Kumar D
Date Deposited: 11 Feb 2016 07:04
Last Modified: 11 Feb 2016 07:04
URI: http://eprints-bangaloreuniversity.in/id/eprint/1016

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